Detection range
N/A
Assay sensitivity
N/A
Reacts with
Human
Conjugation
Biotinylated
Antigen
Biotin protein ligase(HLCS)
ELISA type
find more technical details in the manual
Original name
Human Biotin protein ligase(HLCS) ELISA kit
Test
BlueGen ELISAs supplies other types of Assays as 1.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Reproducibility of the results
Intra-Assay: Coefficient of variability is lower than 10%; Inter-Assay: Coefficient of variability is lower than 15%
Tips
The product Assay kit for human Biotin protein ligase(HLCS) (ELISA) is intended to be used for research purposes only. It is not testesd for application in diagnostics.
Cross reactivity
There is no indicative cross reactivity between the antigen and its analogues detected during the testing of the product Assay kit for human Biotin protein ligase(HLCS) (ELISA)
Product storage
The product Assay kit for human Biotin protein ligase(HLCS) (ELISA) should be kept between two and eight degrees Celsius to ensure the retention of the stability and reactivity of the reagents included in the kit.
Kit configuration
1xMicrotiter test plate; 1 vial x Enzyme conjugate; 1 vial x STANDARD A; 1 vial x STANDARD B; 1 vial x STANDARD C; 1 vial x STANDARD D; 1 vial x STANDARD E; 1 vial x STANDARD F; 1 vial x SUBSTRATE A; 1 vial x SUBSTRATE B; 1 vial x Stop solution; 1 vial x Wash solution (100x); 1 vial x Balance solution; 1 x protocol
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
