human topoisomerases and assays
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For Research Use Only. Produced in USA
ProF supplies other types of Assays as 1.
Reagents for fluorescence-based 384-well or 96-well assays of human topoisomerase I DNA relaxation activity, 20 assays, enzyme is not included
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Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
Human topoisomerase I DNA relaxation assay kit (enzyme not included) includes 160 µl of 10 x Buffer HT1, 84 µl of 10 x supercoiled plasmid DNA, 8 µl of 1500 x Dye H19 and 500 µl of 10 x H19 dilution buffer. It is for 20 DNA relaxation assays in a 96-well plate format or 40 assays in a 384-well assay format. Human topo I enzyme in not included and can be purchased separately.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.