Human kinases and assays
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For Research Use Only. Produced in USA
ProF supplies other types of Assays as 1.
Reagents for fluorescence-based 384-well or 96-well assays of human thymidylate kinase activity, 500 assays
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Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Human Thymidylate Kinase Assay Kit Plus-500 (enzyme included) includes 1700 µl of 10 x reaction buffer, 8 µl of 2500 x human thymidylate kinase (125 uM), 170 µl of 100 x ATP (5 mM), 170 µl of 100 x dTMP (40 mM), 170 µl of 100 x MUK Reagent A, 170 µl of 100 x MUK Reagent B and 1700 µl of 10 x fluorescence dye. The kit reagents are sufficient for 500 thymidylate kinase assays using a standard black 384-well plate (Matrix 4318).